S2657
Sigma
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse
clone SH-B4, ascites fluid
| MDL number: | MFCD00162834 |
Description
| Immunogen | bovine brain S-100b |
| Specificity | Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca2+-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues. |
Properties
| antibody form | ascites fluid |
| clone | SH-B4 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | human, sheep, rabbit, canine, bovine, pig, goat, feline, rat |
| application(s) | dot blot: suitable using denatured-reduced preparations |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using protease-digested, formalin-fixed, paraffin-embedded human tongue sections | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... S100B(6285) |
| rat ... S100b(25742) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Chen, M., et al., Spatiotemporal expression of PSD-95 and nNOS after rat sciatic nerve injury. Neurochem. Res. 33, 1090-1100, (2008) Abstract |
| Helke, C., and Ichikawa, H., Coexistence of s100β and putative transmitter agents in vagal and glossopharyngeal sensory neurons of the rat. Brain Res. 800, 312-318, (1998) Abstract | |
| Janigro, D., A response to Cerebral and extracerebral release of protein S100B in cardiac surgical patients Anaesthesia 59, 1149-1150, (2004) Abstract |
